Enzymes and Inhibitors
Apexbio Technology LLC Phosphatase Inhibitor Cocktail 3 (100X in DMSO) 100x1ml
Numerous cellular processes are regulated by reversible protein phosphorylation with approximately 30% of proteins being phosphorylated during their lifespan Crude cell extracts contain various endogenous enzymes such as phosphatases and proteases which can degrade and alter proteins in the extracts Therefore the optimal method to increase the yield of intact proteins is to add inhibitors targeting these enzymes Phosphatase Inhibitor Cocktail 3 inhibits alkaline phosphatase L-isozymes and serine/threonine protein phosphatases such as protein phosphatase 1 and 2A (PP1 and PP2A) Phosphatase Inhibitor Cocktail 3 has been optimized and tested in cell extracts from various animal tissues Phosphatase Inhibitor Cocktail 3 can replace Phosphatase Inhibitor Cocktail 1 as the two cocktails have equivalent activities Phosphatase Inhibitor Cocktail 3 contains Cantharidin Bromotetramisole and Calyculin A This Phosphatase Inhibitor Cocktail is supplied as a ready-to-use solution in DMSO
New England Biolabs, Inc. StickTogether™ DNA Ligase Buffer – 2 ml
New England Biolabs supplies a reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of T3, T7, Hi-T4 and Salt-T4 DNA Ligases .
Sigma Aldrich Fine Chemicals Biosciences Pyruvate Kinase from rabbi
Pyruvate kinase from rabbit muscle catalyzes an ATP-dependent phosphorylation of glycolate to yield 2-phosphoglycolate.
Revvity Health Sciences Inc OptiScint 3H Quench Standards, 20 mL Vial
OptiScint 3H Quench Standards, 20 mL Vial
Revvity Health Sciences Inc HTRF Enzymatic Kinase Buffer (5X), 10 mL
HTRF Enzymatic Kinase Buffer (5X), 10 mL
Revvity Health Sciences Inc HTRF Stimulation Buffer #2 (5X), 8 mL
HTRF Stimulation Buffer #2 (5X), 8 mL
New England Biolabs, Inc. Exonuclease VIII, truncated – 1000 units
Exonuclease VIII, truncated, is able to initiate nucleotide removal from the 5' termini of linear double-stranded DNA in the 5' to 3' direction.
New England Biolabs, Inc. NEBuffer Set (EcoRI, DpnII) – 1.25 ml of each
New England Biolabs provides 10X NEBuffer with each restriction endonuclease to assure optimal (100%) activity. Set contains two 1.25ml vials of each unique NEBuffer for the enzymes EcoRI and DpnII. Most of our enzymes are supplied with one of four standard NEBuffers. Occasionally, an enzyme has specific buffer requirements not met by one of the four standard NEBuffers, in which case the enzyme is supplied with its own unique NEBuffer. 1X NEBuffer EcoRI 100 mM Tris-HCl 50 mM NaCl 10 mM MgCl2 0.025% Triton X-100 (pH 7.5 @ 25C) 1X NEBuffer DpnII 50 mM Bis-Tris-HCl 100 mM NaCl 10 mM MgCl2 1 mM DTT (pH 6 @ 25C)
Sigma Aldrich Fine Chemicals Biosciences Cellulase enzyme blend
This product contains cellulases AY-glucosidases and hemicellulase for the application of degrading cellulose to fermentable sugars. This product is effective on a wide variety of pre-treated lignocellulosic bimass materials for converting the carbohydrates in these materials into simple sugars prior to fermentation for application in biofuels research.